双色荧光原位杂交检测丙烯腈接触男工精子性染色体数目畸变

doi:10.3969/j.issn.1004-616x.1999.04.004

癌变·畸变·突变 ›› 1999, Vol. 11 ›› Issue (4): 167-171.doi: 10.3969/j.issn.1004-616x.1999.04.004

双色荧光原位杂交检测丙烯腈接触男工精子性染色体数目畸变

徐德祥1 　郑履康2 　邓丽霞2

1安徽医科大学卫生毒理学教研室　合肥　230032 　2中山医科大学卫生毒理学教研室　广州　510089

收稿日期:1998-09-29 修回日期:1999-03-11 出版日期:1999-07-30 发布日期:1999-07-30

STUDY ON NUMERICAL CHROMOSOME ABERRATION IN HUMAN SPERM CELLS ON ACRYLONITRILE2EXPOSED WORKERS USING TWO2COLOR FLUORESCENCE IN SITU HYBRIDIZATION

Xu Dexiang1 , Zheng Lukang2 , Deng Lixia2

1A nhui Medical University Department of Toxicology , Hef ei 　230032 　2 Sen Yat2sen University of Medical Science , Department of Toxicology , Guangz hou 　510089

Received:1998-09-29 Revised:1999-03-11 Online:1999-07-30 Published:1999-07-30

摘要/Abstract

摘要： 本研究用荧光原位杂交技术(FISH) 对9 名丙烯腈接触男工和9 名健康者精子性染色体数目畸变率进行了检测,结果显示,精子性染色体杂交点荧光信号强,背景清楚,在镜下容易观察;接触组和对照组杂交效率均大于99 %。9 名对照者X 和Y精子分别为4911 %和4916 % ,X、Y精子比例接近于1∶1 。对照人群精子性染色体数目畸变率与其它试验室检出结果基本一致。接触组和对照组分别共计数了91015 和74679 个精子细胞核,两组精子性染色体数目畸变率分别为0. 70 %和0. 35 % ,有显著性差异;其中接触组XX、YY、XY分别为0. 10 %、0. 23 %、0. 37 % ,明显高于对照组的0. 05 %、0110 %和0120 % ,表明使用荧光原位杂交技术(FISH) 能检出接触丙烯腈引起的精子性染色体数目畸变。

关键词: 丙烯腈, 精子, 性染色体, 非整倍体, 荧光原位杂交

Abstract: In this study , Fluorescence in situ hybridization (FISH) was used to investigate rates of sex chromosome aneuploidy in spermatozoa among 9 acrylonit rile2exposed workers and 9 healthy sperm donors. 74679 sperm nuclei were counted among 9 healthy sperm donors , and 91015 sperm nuclei among 9 acrylonit rileexposed workers. The average f requency of total chromosome numerical abnormalities was 0. 70 % in exposure group , and 0. 35 % in the cont rol. XY2bearing sperm was the most common sex chromosome disomy , with an average rate of 0. 37 % in exposure group , and 0. 20 % in the cont rol. XX2and YY2bearing sperm accounted for an additional 0. 10 % and 0. 23 % in exposure group , and 0. 05 % and 0. 10 % in the cont rol. Significantly increased f requencies of total sex chromosome disomy , and XX2 ,YY2and XY2bearing sperm were observed among acrylonit rile2exposed workers , as compared with healthy sperm donors. This study demonst rated that sperm FISH was a useful biomarker to detect and compare numerical chromosome aberration in human sperm cells for heritable risk assessment of acrylonit rile.

Key words: Aneuploidy, Sex Chromosome, Sperm, Fluorescence In Situ Hybridization (FISH), Acrylonit rile

徐德祥　郑履康　邓丽霞. 双色荧光原位杂交检测丙烯腈接触男工精子性染色体数目畸变[J]. 癌变·畸变·突变, 1999, 11(4): 167-171.

Xu Dexiang , Zheng Lukang , Deng Lixia. STUDY ON NUMERICAL CHROMOSOME ABERRATION IN HUMAN SPERM CELLS ON ACRYLONITRILE2EXPOSED WORKERS USING TWO2COLOR FLUORESCENCE IN SITU HYBRIDIZATION[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 1999, 11(4): 167-171.

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双色荧光原位杂交检测丙烯腈接触男工精子性染色体数目畸变

STUDY ON NUMERICAL CHROMOSOME ABERRATION IN HUMAN SPERM CELLS ON ACRYLONITRILE2EXPOSED WORKERS USING TWO2COLOR FLUORESCENCE IN SITU HYBRIDIZATION

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