关键词: Annexin V, PI, 胃癌, 细胞早期凋亡, 定量分析

Abstract: Purpose : Measuring the amounts of fluorescence by bivariate flow cytomet ry ( FCM) , we examined early stage of apoptosis in 6 vicious gast ric tissues and corresponding normal ones with a double staining method of Annexin V(AV) and propidiom iodide (PI) . Methods : From 6 vicious and 6 normal gast ric tissues of 1cm3 ,single2cell suspension were preparated and stained with fluorescein isothiocyanate ( FITC)2labelled AV and PI ,and AV binding was assessed using bivariate FCM. Results : The separated cells incubated with AV and PI enabled three distinct populations of cells(apoptotic , necrotic and living cells) to be identified and sorted by FCM.Comparing normal and vicious tissues , differences were great among the three populations of cells. Conclusion :The AV/ PI bivariate FCM allows the possibility of early phase of apoptosis in early gast ric carcinoma , which offers a method to research clinically gast ric carcinoma.

Key words: Annexin V, propidiom iodide, gast ric carcinoma, early stage of apoptosis, quantification