癌变·畸变·突变 ›› 2004, Vol. 16 ›› Issue (3): 151-154.doi: 10.3969/j.issn.1004-616x.2004.03.008

• 论著 • 上一篇    下一篇

三价铬和六价铬对小鼠骨髓细胞微核率和精子畸变影响的比较

考庆君，吴　坤,李红卫，蔺玉华，姜丽英   

  1. 哈尔滨医科大学公共卫生学院，黑龙江　哈尔滨　150001
  • 收稿日期:2004-01-05 修回日期:2004-03-15 出版日期:2004-05-30 发布日期:2004-05-30
  • 通讯作者: 吴　坤

The Effect of Trivalent Chromium and Hexavalent Chromium on the Micronuclear Rate and the Sperm Abnormality of Mice

KAO Qing-jun，WU Kun，LI Hong-wei，et al   

  1. Public Health college,Harbin Medical University,Harbin 150001，China
  • Received:2004-01-05 Revised:2004-03-15 Online:2004-05-30 Published:2004-05-30
  • Contact: WU Kun

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摘要： 背景与目的：比较不同价态铬化合物对小鼠体细胞和雄性生殖细胞的遗传毒性。材料与方法：小鼠骨髓嗜多染红细胞微核实验和小鼠精子畸变分析。结果：小鼠骨髓微核实验，分别以1/5 LD50、1/10 LD50、1/20 LD50作为高、中、低剂量组。CrCl3各剂量组微核率与对照组相比均差异无显著性；K2Cr2O7各剂量组微核率与对照组相比明显增高，差异有显著性。CrCl3和K2Cr2O7各剂量组骨髓细胞PCE/RBC比值随剂量的增高均呈现下降趋势，但均高于0.1；小鼠精子畸变分析试验，分别1/2 LD50、1/4 LD50、1/8 LD50为高、中、低剂量组。CrCl3和K2Cr2O7各剂量组精子畸形率与对照组相比均明显增高，差异有显著性意义。结论：CrCl3对体细胞染色体无损伤作用，但对雄性生殖细胞具有遗传毒性；K2Cr2O7对体细胞和雄性生殖细胞均不具有毒性作用。在相同的致死效应剂量下，K2Cr2O7对体细胞和雄性生殖细胞的损伤程度要明显高于CrCl3。

关键词: 铬, 微核, 精子畸变

Abstract: BACKGROUND ＆ AIM：To evaluate the toxic effect of chromium to the boby cell and the sperm of mice. MATERIAL AND METHODS： The micronuclear test in polychromatic erythrocytes (PCES) of bone marrow and the sperm abnormality test were applyed in mice model. RESULTS: For the micronuclear test，use 1/5 LD50、1/10 LD50、1/20 LD50 as the high,middle,and low dosage respectively.The results showed that CrCl3 had no significant effect on the micronuclear rate in treatment groups and control group(P>0.05).While K2Cr2O7 could dramatically increase the micronuclear rate ,which was significantly higher than that of control group(P<0.01).the ratio of PCE/RBC in every group presented in reverse relationship with the dosage of CrCl3 and K2Cr2O7,but higher than 0.1.For the sperm abnormality test，use 1/2 LD50、1/4 LD50、1/8 LD50 as the high,middle,and low dosage respectively.The sperm abnormality rate of CrCl3 and K2Cr2O7 treatment groups were significantly higher than that of control group(P<0.05). CONCLUSION: CrCl3 have no toxicity on the boby cell and might have genotoxicity effect on the sperm of mice.K2Cr2O7 has significantly toxicity on the boby cell and sperm of mice. With the same dosage of lethal effect，the toxicity effect of K2Cr2O7 is significantly stronger than that of CrCl3 .

Key words: chromium, micronuclear, sperm abnormality

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