癌变·畸变·突变 ›› 2006, Vol. 18 ›› Issue (4): 257-260.doi: 10.3969/j.issn.1004-616x.2006.04.001

• 论著 •    下一篇

溴氰菊酯对大鼠脑组织线粒体膜电位和膜流动性的影响

陈　丹;石　年;李煌元;刘琳琳   

  1. 华中科技大学同济医学院公共卫生学院卫生毒理学系，环境与健康教育部重点实验室湖北　武汉,430030
  • 收稿日期:2005-09-12 修回日期:2005-12-19 出版日期:2006-07-30 发布日期:2006-07-30
  • 通讯作者: 石　年

Effects of Deltamethrin on Mitochondrial Membrane Potential and Membrane Fluidity in Rats Brain Tissue

CHEN Dan, SHI Nian, LI Huang-yuan, LIU Lin-lin   

  1. Department of Health Toxicology, Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430030, Hubei，China
  • Received:2005-09-12 Revised:2005-12-19 Online:2006-07-30 Published:2006-07-30
  • Contact: Shi Nian

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摘要： 背景与目的： 研究溴氰菊酯(Deltamethrin,DM)对大鼠脑组织线粒体膜电位及膜流动性的影响。材料与方法： 成年雄性Wistar大鼠一次性腹腔注射12.5 mg/kg体重DM(溶剂为色拉油)，5 h、24 h、48 h、72 h后处死，提取皮层和海马的线粒体，分别测定线粒体膜电位、膜流动性、Na＋-K＋、Ca2＋-Mg2＋-ATP酶和琥珀酸脱氢酶活力。同时设立对照组，只注射色拉油0.5 ml/100 g，5 h后处死大鼠。 结果： 大鼠经DM处理后，5 h、24 h、48 h、72 h组的线粒体膜电位下降，膜流动性降低，Na＋-K＋、Ca2＋-Mg2＋-ATP酶和琥珀酸脱氢酶活力受到抑制，与对照组相比，差异均具有统计学意义(P<0.01)，并且与染毒后时间成直线相关关系。 结论： DM能明显损害大鼠脑组织线粒体功能，继而引起线粒体氧化磷酸化障碍。

关键词: 溴氰菊酯, 线粒体, 膜电位, 膜流动性

Abstract: BACKGROUND ＆ AIM: To study the effects of deltamethrin(DM) on mitochondrial membrane potential and membrane fluidity in rat brain tissue. MATERIAL AND METHODS: Male adult Wistar rats were treated intraperitonealy with 12.5 mg/kg DM. At 5, 24, 48 and 72 h after injection, we extracted the mitochondria of rat brain tissue to measure the membrane potential, membrane fluidity, activities of Na＋-K＋-ATPase、Ca2＋-Mg2＋-ATPase and succinic dehydrogenase.At the same time the control group were merely injected intraperitonealy with 0.5 mg/100 g salad oil and then executed after 5 h. RESULTS: After treatment with deltamethrin, the mitochondrial membrane potential decreased, membrane fluidity was reduced, the activities of Na＋-K＋-ATPase,Ca2＋-Mg2＋-ATPase and succinic dehydrogenase were inhibited (P<0.01). Moreover, there were correlations between those indexes and time after treatment. CONCLUSION: DM had obvious effects on inhibiting the mitochondrial function of rat brain tissue, then caused the impairment of oxidative phosphorylation in the mitochondria.

Key words: deltamethrin, mitochondria, membrane potential, membrane fluidity

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