绿茶对微囊藻毒素LR诱导肝细胞凋亡、Bcl-2表达及骨髓嗜多染红细胞微核的影响

doi:10.3969/j.issn.1004-616x.2007.01.009

癌变·畸变·突变 ›› 2007, Vol. 19 ›› Issue (1): 29-032.doi: 10.3969/j.issn.1004-616x.2007.01.009

绿茶对微囊藻毒素LR诱导肝细胞凋亡、Bcl-2表达及骨髓嗜多染红细胞微核的影响

许　川1；舒为群1；邱志群1；常晓松1；罗教华1；付文娟1；曹　佳2

1. 第三军医大学军事预防医学院环境卫生学教研室； 2.第三军医大学军事预防医学院卫生毒理学教研室，重庆　400038)

收稿日期:2006-09-04 修回日期:2006-09-04 出版日期:2007-01-30 发布日期:2007-01-30

Effect of Green Tea on Microcystin-LR-induced Hepatocelluar Apoptosis, Bcl-2 Expression and Micronucleus

XU Chuan1, SHU Wei-qun1, QIU Zhi-qun1, CHANG Xiao-song1, LUO Jiao-hua1, FU Wen-juan1, CAO Jia2

1. Department of Environmental Hygiene;2. Department of Hygiene Toxicology,School of Military Preventive Medicine, The Third Military Medical University, Chongqing 400038,China)

Received:2006-09-04 Revised:2006-09-04 Online:2007-01-30 Published:2007-01-30

摘要/Abstract

摘要： 背景与目的: 研究绿茶(green tea,GT)对微囊藻毒素LR(MC-LR)诱导肝细胞凋亡、Bcl-2蛋白表达及微核发生的影响以探讨毒性拮抗机制。材料与方法: 雄性小鼠50只随机分为5组，分别为空白对照、MC-LR染毒组、GT高低剂量拮抗组和环磷酰胺对照组。实验第1 d起GT高、低剂量拮抗组小鼠每日分别给予12 g/L和2 g/L两种浓度的GT自由饮用，连续18 d。自第6 d开始，染毒小鼠每日给予MC-LR 10 μg/kg腹腔注射1次，空白对照给予DMSO腹腔注射，连续13 d。环磷酰胺对照组以50 mg/kg剂量间隔24 h两次给药后6 h取材。小鼠处死后采用免疫组化和计数法对肝细胞凋亡、Bcl-2蛋白表达以及骨髓嗜多染红细胞(PCEs)微核发生率进行检测和分析。结果: (1) MC-LR染毒明显诱导小鼠肝细胞凋亡增加。高剂量GT处理后明显抑制MC-LR染毒所致小鼠肝细胞凋亡的发生(P<0.05)；(2) 单纯MC-LR染毒肝细胞Bcl-2表达未见明显变化，GT各剂量组小鼠肝脏Bcl-2的表达明显增加，与MC-LR染毒组相比差异具有统计学意义(P<0.01)。(3) GT拮抗组小鼠骨髓嗜多染红细胞微核率(PCEs-MN) 与MC-LR染毒对照和空白对照相比，其差异均无统计学意义(P>0.05)。结论: GT能上调抑癌基因Bcl-2的表达，抑制细胞凋亡。MC-LR染毒及GT拮抗对微核发生均未有显著影响。

关键词: 绿茶, 微囊藻毒素LR, 凋亡, Bcl-2, 微核

Abstract: BACKGROUND ＆ AIM： To evaluate the effects of green tea (GT) on Microcystin-LR (MC-LR)-induced hepatocelluar apoptosis, Bcl-2 expression and micronucleus test so as to explore antagonistic mechanism of GT. MATERIALS AND METHODS： 50 male mice were randomly divided into five groups. Mice in GT pretreated groups were given green tea as free drink at doses of 2 g/(L·d) and 12 g/(L·d) prior to MC-LR intoxication, consecutively for 18 days. The toxin treatment mice in group MC-LR control received continual intraperitoneal injections of MC-LR at dose of 10 μg/(kg·d) for 13 days from day 6 till sacrifice. CP control group was treated with intraperitoneal cyclophosphamide twice at dose of 50 mg/(kg·d) 24 h interval on days 17 and 18. Mice were sacrificed and immediately subjected to autopsy. Hepatocellular apoptosis, Bcl-2 protein expression and micronucleus frequencies of bone marrow were evaluated immediately. RESULTS： (1) MC-LR induced obvious hepatocellular apoptosis. High dose of GT pretreatment significantly inhibited MC-LR-induced hepatocellular apoptosis(P<0.05).(2) There was no significant change of Bcl-2 protein expression in MC-LR control compared with control. As compared with only MC-LR, the expression of Bcl-2 protein was significantly increased in GT pretreatment groups(P<0.01).(3) No significant difference in micronucleus frequencies was found in either MC-LR control or GT pretreatment groups compared with control (P>0.05). CONCLUSION： GT could increase Bcl-2 protein expression and inhibit hepatocellular apoptosis，MC-LR and GT pretreatment did not cause damage to mice chromosome.

Key words: green tea, microcystin-LR, apoptosis, bcl-2, micronucleus

中图分类号:

R994.4

许　川, 舒为群, 邱志群, 常晓松, 罗教华, 付文娟, 曹　佳. 绿茶对微囊藻毒素LR诱导肝细胞凋亡、Bcl-2表达及骨髓嗜多染红细胞微核的影响[J]. 癌变·畸变·突变, 2007, 19(1): 29-032.

XU Chuan, SHU Wei-qun, QIU Zhi-qun, CHANG Xiao-song, LUO Jiao-hua, FU Wen-juan, CAO Jia. Effect of Green Tea on Microcystin-LR-induced Hepatocelluar Apoptosis, Bcl-2 Expression and Micronucleus[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2007, 19(1): 29-032.

[1]	李柏茹, 秦双建, 关岚, 李闰冰, 蔡颖, 蒲桔宁, 曾明, 肖芳, 徐新云. PM_2.5对HK-2细胞氧化损伤和凋亡的影响[J]. 癌变·畸变·突变, 2021, 33(5): 360-364.
[2]	蔡绮, 高德煜, 史建峰, 于浩, 韩妍, 杨柳, 赵蕾, 王召旭. 以树鼩为微核试验新型动物模型的初步研究[J]. 癌变·畸变·突变, 2021, 33(5): 370-374,382.
[3]	黄晓华, 牛永东, 石刚刚. 核受体FXR激活对宫颈癌CaSki细胞增殖的抑制效应及其作用机制[J]. 癌变·畸变·突变, 2021, 33(4): 302-306.
[4]	王熙凯, 孟庆贺, 高燕鲁. miR-223-3p通过调控ECT2诱导非小细胞肺癌细胞凋亡的实验研究[J]. 癌变·畸变·突变, 2021, 33(3): 187-192.
[5]	陆雪, 田雪蕾, 赵骅, 蔡恬静, 田梅, 刘青杰. 人外周血淋巴细胞微核分析用于估算离体模拟局部照射剂量的研究[J]. 癌变·畸变·突变, 2021, 33(3): 193-196.
[6]	李柏茹, 秦双建, 李闰冰, 蔡颖, 郑凯, 曾明, 肖芳, 徐新云. p38MAPK基因对PM_2.5染毒HK-2细胞部分癌基因和凋亡相关基因表达的影响[J]. 癌变·畸变·突变, 2021, 33(2): 129-135,142.
[7]	马顺鹏, 王全凯, 王苗, 宋佳阳, 乌瀚宝栎尔, 谢广云, 许建宁. 甲基丙烯酸环氧丙酯诱导16HBE细胞恶性转化过程中细胞凋亡相关lncRNA的筛选及功能研究[J]. 癌变·畸变·突变, 2021, 33(1): 1-5,31.
[8]	李柏茹, 秦双建, 李闰冰, 蔡颖, 郑凯, 王冰玉, 曾明, 肖芳, 徐新云. PM_2.5对HK-2肾细胞部分癌基因和凋亡基因表达的影响[J]. 癌变·畸变·突变, 2021, 33(1): 6-11.
[9]	黄立利, 张梦云, 刘科亮, 庞定国, 刘丽达, 徐培渝. 2,4-二氯苯氧乙酸对幼龄SD大鼠肝毒性的研究[J]. 癌变·畸变·突变, 2021, 33(1): 22-27.
[10]	崔昭阳, 李素娜, 姜旭倩, 王弈丹, 侯丽颖. 芹菜素激活TRAIL死亡受体参与诱导胃癌SGC-7901细胞凋亡的实验研究[J]. 癌变·畸变·突变, 2021, 33(1): 32-36.
[11]	赵骅, 陆雪, 蔡恬静, 田梅, 刘青杰. 年龄和性别对正常人外周血淋巴细胞核质桥自发率的影响[J]. 癌变·畸变·突变, 2021, 33(1): 48-52.
[12]	兰莉, 陈海丽, 徐蕾. RBMS3-AS3对宫颈癌细胞增殖、凋亡和侵袭的影响及作用机制[J]. 癌变·畸变·突变, 2020, 32(6): 438-443.
[13]	刘鹏, 白意晓. miR-29a对人胃癌细胞增殖、迁移的影响及其机制研究[J]. 癌变·畸变·突变, 2020, 32(6): 452-456,463.
[14]	杨兴肖, 张雪原, 邹乃祎, 单保恩, 马鸣, 祝淑钗. HMGB1 siRNA干扰对食管鳞癌细胞放射敏感性的影响[J]. 癌变·畸变·突变, 2020, 32(4): 269-274.
[15]	秦双建, 李柏茹, 蔡颖, 郑凯, 王冰玉, 李闰冰, 肖芳, 曾明, 徐新云. PM_2.5对L02肝细胞部分癌基因和凋亡相关基因表达的影响[J]. 癌变·畸变·突变, 2020, 32(4): 281-285.

绿茶对微囊藻毒素LR诱导肝细胞凋亡、Bcl-2表达及骨髓嗜多染红细胞微核的影响

Effect of Green Tea on Microcystin-LR-induced Hepatocelluar Apoptosis, Bcl-2 Expression and Micronucleus

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价