癌变·畸变·突变 ›› 2007, Vol. 19 ›› Issue (5): 351-354.doi: 10.3969/j.issn.1004-616x.2007.05.002

• 论著 • 上一篇    下一篇

非霍奇金淋巴瘤患者红细胞CR1基因多态性、表达数量及免疫功能的相关性

刘丽宏1/ 单保恩2/ 高玉环1/ 潘　崚3,   

  1. (1.河北医科大学第四医院血液科；2. 河北医科大学第四医院科研中心，河北　石家庄　050011；3.河北医科大学第二医院血液科，河北　石家庄　050011)
  • 收稿日期:2007-05-10 修回日期:2007-06-20 出版日期:2007-09-30 发布日期:2007-09-30
  • 通讯作者: 潘　崚

Relationship Between CR1 on Erythrocyte Genomic Poly_morphism with Expression of ECR1 and the Immune Function in Patients with NHL

LIU Li_hong, SHAN Bao_en2, GAO Yu_huan, PAN Ling3,   

  1. (1.Department of Hematology,2.Research central,The Fourth hospital,3.Department of hematology,the second hospital, of Hebei Medical University, Shijiazhuang 050011,Hebei, China)
  • Received:2007-05-10 Revised:2007-06-20 Online:2007-09-30 Published:2007-09-30
  • Contact: PAN Ling

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摘要： 背景与目的：通过研究非霍奇金淋巴瘤(NHL)患者红细胞补体受体一型分子(CR1) 基因多态性分布、表达数量及与免疫功能的相关性，探讨淋巴瘤患者的免疫功能变化。 材料与方法： 对60名正常人和96例非霍奇金淋巴瘤患者，采用PCR和Hind Ⅲ酶切技术以及酶联法定量测定红细胞CR1基因多态分布和分子的数量，采用郭峰法测定红细胞免疫功能，ELISA法测定血清中CD4、CD8水平。 结果： 非霍奇金淋巴瘤患者红细胞CR1分子密度相关基因高表达型较正常人明显下降,中、低表达型明显升高(P<0.01)。非霍奇金淋巴瘤患者CR1分子的表达数量低于正常健康人水平(P<0.01)。CR1基因多态分布与CR1分子表达数量及红细胞、淋巴细胞免疫功能明显相关。 结论： 非霍奇金淋巴瘤患者红细胞CR1密度基因多态性及分子表达数量与机体免疫功能变化相关，可能是影响淋巴瘤预后的指标。

关键词: 淋巴瘤, 非霍奇金, CR1, 基因多态性, 免疫

Abstract: BACKGROUND ＆ AIM: To study the relationship between CR1 genomic density polymorphism of erythrocyte with the quantitative expression of ECR1 and immune functions in patients with Non_Hodgkin Lymphoma. MATERIALS AND METHODS: ECR1 gene in erythrocytes was measured with PCR_RFLP method.Expression level of ECR1 was assessed by the quantitative assay. Soluble CD4, CD8 levels in serum were detected by ELISA. The RBC_C3bRR, RBC_ICR and TRR levels in erythrocyte were detected by the method established by Guo Feng. RESULTS: Expression of ECR1 gene in erythrocytes of Non_Hodgkin Lymphoma patients was markedly lower than that in normal persons(P<0.01), but HL type and LL type were markedly higher than that of normal people(P<0.01). The expression level of ECR1 of Non_Hodgkin Lymphoma patient was markedly lower than normal persons(P<0.01). There was positive correlation between CR1 genomic density polymorphism and expression level of ECR1 in erythrocytes and lymphocytic immune function. CONCLUSION：There was positive correlation between CR1 genomic density polymorphism with expression level of ECR1 in erythrocyte and immune function in the body. That could affect the prognosis of patients with Non_Hodgkin.

Key words: lymphoma, Non_Hodgkin, CR1, polymorphism, immune

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