关键词: PBDE_47, 致突变, 微核, 核浆桥

Abstract: BACKGROUND ＆ AIM: To study the mutagenicity of 2,2＇,4,4＇_tetrabromodiphenyl ethers (PBDE_47) in human neuroblastoma SH_SY5Y cells in vitro. MATERIALS AND METHODS: The frequencies of micronuclei(MNi) and nucleoplasmic bridges (NPBs) were measured after SH_SY5Y cells were exposed to different doses of PBDE_47 for 24 h in vitro. RESULTS: PBDE_47 caused a significant concentration_dependent decrease in nuclear division index(NDI), and a concentration_dependent increase in MNi frequency in terms of total MNi/1000 binucleated cells (BNCs), BNCs with MNi/1000 BNCs, and NPBs/1000 BNCs. The NDI was obviously decreased, while the frequencies of BNCs with MNi/1000 BNCs and NPBs/1000 BNCs were increased significantly at 2 μg/ml and above PBDE_47 _treated groups (P<0.05). There was statistically difference in frequcency of MNi/1000 BNCs only in 4 μg/ml PBDE_47_treated group (P<0.05). CONCLUSION: PBDE_47 may induce the MNi and NPB formation, causing mutagenicity.

Key words: PBDE_47, mutagenicity, micronuclei, nucleoplasmic bridges