癌变·畸变·突变 ›› 2010, Vol. 22 ›› Issue (4): 276-278.doi: 10.3969/j.issn.1004-616x.2010.04.006

• 论著 • 上一篇    下一篇

氯化镉致人肝癌细胞SMMC-7721线粒体损伤作用

刘晓梅1;石磊1;王雯1;金明华1;杜海英1;刘颖1;孙磊1;孙志伟1;2   

  1. 1. 吉林大学公共卫生学院，吉林 长春 130021； 2. 首都医科大学公共卫生与家庭医学学院，北京 100069
  • 收稿日期:2009-10-20 修回日期:2010-01-30 出版日期:2010-07-30 发布日期:2010-07-30
  • 通讯作者: 孙志伟

Mitochondrial damage in SMMC-7721 cells induced by cadmium chloride

LIU Xiao-mei1;SHI Lei1;WANG Wen1;JIN Ming-hua1;DU Hai-ying1;LIU Ying1;SUN Lei1;SUN Zhi-wei1;2   

  1. 1. School of Public Health, Jilin University, Changchun 130021； 2. School of Public Health and Family Medicine, Capital Medical University, Beijing 100069,China
  • Received:2009-10-20 Revised:2010-01-30 Online:2010-07-30 Published:2010-07-30
  • Contact: SUN Zhi-wei

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摘要： 目的： 研究氯化镉对人肝癌细胞SMMC-7721线粒体的损伤作用。 方法： 分别用5、10、20、30、40 μmol/L氯化镉作用体外培养的SMMC-7721细胞24 h，实验同时设对照组。采用形态学方法检测其线粒体的结构完整性，用分光光度法检测线粒体钙离子转运能力，采用琼脂糖凝胶电泳法检测线粒体DNA损伤。 结果： 随着氯化镉的浓度升高，存活细胞数降低，细胞内的线粒体数量减少；氯化镉处理组细胞线粒体钙离子转运能力下降，并可见线粒体DNA损伤条带。 结论： 氯化镉可以引起人肝癌细胞SMMC-7721线粒体及线粒体DNA损伤。

关键词: 氯化镉, SMMC-7721, 线粒体, 钙离子

Abstract: OBJECTIVE: The mitochondrial damage of SMMC-7721 cells induced by cadmium chloride was studied in vitro. METHODS: The SMMC-7721 cells were treated with cadmium chloride for 24 hours. Morphology was used to assess integrity of mitochondria. Spectrophotography was used to measure the transport ability of Ca2＋ into mitochondria and agarose gel electrophoresis was used to observe the mitochondrial DNA damage. RESULTS: The mumber of treated cell and mitochondria decreased with increasing cadmium concentration. The transport ability of Ca2＋ into mitochondria was decreased and the mitochondrial DNA was damaged with increasing concentration. CONCLUSION: Cadmium chloride could damage the mitochondria and its DNA in SMMC-7721 cells.

Key words: cadmium chloride, SMMC-7721, mitochondria, calciumion

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