›› 2012, Vol. 24 ›› Issue (4): 298-301.doi: 10.3969/j.issn.1004-616x.2012.04.014

• 检测研究 • 上一篇    下一篇

人参皂苷Rb2致中国仓鼠肺细胞染色体畸变的作用

高 梅;曹 冲;朱春花;曲保恩   

  1. (山东省医药工业研究所，山东省化学药物重点实验室，山东 济南 250101 )
  • 收稿日期:2012-02-20 修回日期:2012-05-23 出版日期:2012-07-30 发布日期:2012-07-30
  • 通讯作者: 高 梅

Effects of ginsenoside Rb2 on chromosome aberration in Chinese hamster lung cells

GAO Mei;CAO Chong;ZHU Chun-hua;QU Bao-en   

  1. (Institure of Pharmaceutical Industry，Shandong Provincial Key Laboratory of Chemical Drug，Jinan 250101，Shangdong, China)
  • Received:2012-02-20 Revised:2012-05-23 Online:2012-07-30 Published:2012-07-30
  • Contact: GAO Mei

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摘要： 目的：研究人参皂苷Rb2致中国仓鼠肺细胞(Chinese hamster lung cells，CHL)染色体畸变的作用。方法：细胞计数法测定人参皂苷Rb2对CHL细胞的半数抑制浓度(50% inhibition concentration，IC50)，根据IC50设立不同剂量组，进行CHL细胞染色体畸变试验，分别观察人参皂苷Rb2染毒6、24 h及加S9后染毒6 h CHL细胞染色体的数目及结构变化，进行染色体畸变分析。结果：人参皂苷Rb2染毒6、24 h及加S9后染毒6 h CHL细胞染色体畸变均为阴性。结论：在本试验条件下，人参皂苷Rb2未引起CHL细胞染色体产生畸变。

关键词: 人参皂苷Rb2, 中国仓鼠肺细胞, 染色体畸变

Abstract: OBJECTIVE: To explore the effects of ginsenoside Rb2 on chromosome aberration in Chinese hamster lung(CHL) cells. METHODS：We used the method of cell counting to determine the IC50 of ginsenoside Rb2 on CHL cells，then established the range of doses according to the IC50 and performed chromosome aberration. The results were based on the chromosome number and structural changes. RESULTS：Negative response was found at 6 h and 24 h after treatment with ginsenoside Rb2 and at 6 h after the addition of S9 mixture. CONCLUSION：The ginsenoside Rb2 did not induce chromosome aberration in CHL cells under our experimental conditions.

Key words: ginsenoside Rb2, Chinese hamster lung cells, chromosome aberration

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