协同刺激分子B7-H4在食管癌前病变组织中的表达及临床意义

doi:10.3969/j.issn.1004-616x.2018.05.008

癌变·畸变·突变 ›› 2018, Vol. 30 ›› Issue (5): 368-373.doi: 10.3969/j.issn.1004-616x.2018.05.008

协同刺激分子B7-H4在食管癌前病变组织中的表达及临床意义

陈欣然¹, 宋永志², 单保恩³

1. 河北医科大学第四医院药学部, 河北石家庄 050011;
2. 河北省医学情报研究所情报研究室, 河北石家庄 050021;
3. 河北医科大学第四医院科研中心, 河北石家庄 050011

收稿日期:2018-03-17 修回日期:2018-09-09 出版日期:2018-09-30 发布日期:2018-09-30
通讯作者: 单保恩,E-mail:hbthshan@163.com E-mail:hbthshan@163.com
作者简介:陈欣然,E-mail:cxrtht@163.com。
基金资助:
河北省科技计划项目（162777174）

Clinical significance in expression of costimulatory molecule B7-H4 in precancerous esophageal tissues

CHEN Xinran¹, SONG Yongzhi², SHAN Baoen³

1. Department of Pharmacy, Fourth Hospital of Hebei Medical University, Shijiazhuang 050011;
2. Information Research Office of Hebei Medical Information Institute, Shijiazhuang 050021;
3. Research Center, Fourth Hospital of Hebei Medical University, Shijiazhuang 050011, Hebei, China

Received:2018-03-17 Revised:2018-09-09 Online:2018-09-30 Published:2018-09-30

摘要/Abstract

摘要： 目的：研究协同刺激分子B7同系物4（B7-H4）在人食管癌前病变组织中的表达变化，探讨其在食管鳞状细胞癌形成中的临床意义。方法：收集内镜切除的食管组织标本58例，其中正常组织16例，低级别上皮内瘤变（LGIN）23例，高级别上皮内瘤变（HGIN）19例。通过苏木素-伊红（HE）染色评价食管组织病理变化。分别采用实时荧光定量PCR（qPCR）检测B7-H4 mRNA在食管组织的表达；免疫组织化学和Western blot检测B7-H4蛋白在食管病变组织中的表达；酶联免疫吸附法（ELISA）检测食管组织细胞因子IL-6、IL-10和IFN-γ的表达，并分析各细胞因子与B7-H4蛋白表达的相关性。结果：免疫组化和HE染色结果提示，B7-H4表达与食管组织病理分级呈显著正相关（P < 0.01）。qPCR结果提示，与正常食管组织相比，食管癌前病变组织B7-H4 mRNA表达无显著改变（P > 0.05）。而Western blot实验结果显示，与正常食管组织相比，食管癌前病变组织B7-H4蛋白表达显著升高（P < 0.05）。Westernblot和ELISA实验结果显示B7-H4表达与IL-6表达呈正相关（P < 0.01）。结论：随着食管癌前病变病理分级的提高，B7-H4蛋白的表达逐渐上调，B7-H4可能与IL-6相互作用促进了食管鳞状细胞癌的发生发展。

关键词: 食管鳞状细胞癌, B7-H4, 食管癌前病变, IL-6

Abstract: OBJECTIVE:To study expression of a costimulatory molecule, B7 homologue 4 (B7-H4), in precancerous lesions of human esophageal carcinoma and to understand its clinical significance in the formation of esophageal squamous cell carcinoma. METHODS:58 esophageal tissue specimens of endoscopic resection were collected. There were 16 cases of normal tissues,23 cases of low-grade intraepithelial neoplasia (LGIN) and 19 cases of high-grade intraepithelial neoplasia (HGIN). Hematoxylin eosin staining (HE) was used to evaluate their pathological changes. Expression of B7-H4 mRNA in esophageal tissues was detected by real-time fluorogenic quantitative-PCR (qPCR). Immunohistochemistry and Immunoblotting (Western blot) methods were used to detect B7-H4 protein expression in esophageal tissues. Expressions of IL-6, IL-10 and IFN-γ in esophagi were detected by Enzyme-linked Immunosorbent assay (ELISA). Additionally,correlations between expression of these cytokines and B7-H4 protein were analyzed. RESULTS:Immunohistochemistry and HE results indicate that B7-H4 protein expression was positively correlated with esophageal pathological grade (P < 0.01). Moreover,the qPCR results show that there was no significant difference in expression of B7-H4 mRNA between precancerous tissues and normal esophageal tissues (P > 0.05). Western blot data indicate that B7-H4 protein expression was increased significantly in precancerous esophagi compared with that of normal esophagi (P < 0.05). Furthermore,results of Western blot and ELISA tests show that B7-H4 expression was positively correlated with IL-6 concentration (P < 0.01). CONCLUSION:B7-H4 protein expression was gradually upregulated with the increase of precancerous grade of esophagi. B7-H4 may promote esophageal squamous cell carcinoma formation by interacting with IL-6.

Key words: esophageal squamous cell carcinmoma, B7-H4, esophageal precancerous conditions, IL-6

中图分类号:

R735.1

陈欣然, 宋永志, 单保恩. 协同刺激分子B7-H4在食管癌前病变组织中的表达及临床意义[J]. 癌变·畸变·突变, 2018, 30(5): 368-373.

CHEN Xinran, SONG Yongzhi, SHAN Baoen. Clinical significance in expression of costimulatory molecule B7-H4 in precancerous esophageal tissues[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2018, 30(5): 368-373.

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协同刺激分子B7-H4在食管癌前病变组织中的表达及临床意义

Clinical significance in expression of costimulatory molecule B7-H4 in precancerous esophageal tissues

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