基于生物信息学方法分析甲酰肽受体1在胶质瘤中的表达及临床意义

doi:10.3969/j.issn.1004-616x.2018.06.008

癌变·畸变·突变 ›› 2018, Vol. 30 ›› Issue (6): 457-462.doi: 10.3969/j.issn.1004-616x.2018.06.008

基于生物信息学方法分析甲酰肽受体1在胶质瘤中的表达及临床意义

董强¹, 解琪琪², 胡建宏¹, 王茂林¹, 袁国强³, 潘亚文^1,3

1. 兰州大学第二医院神经外科, 甘肃兰州 730030;
2. 兰州大学第二医院骨科, 甘肃兰州 730030;
3. 兰州大学第二医院神经病学研究所, 甘肃兰州 730030

收稿日期:2018-06-27 修回日期:2018-10-14 出版日期:2018-11-30 发布日期:2018-11-30
通讯作者: 潘亚文,E-mail:panyw2018@163.com E-mail:panyw2018@163.com
作者简介:董强,E-mail:dongq16@lzu.edu.cn。
基金资助:
国家自然科学基金项目（81771297）；甘肃省卫生行业科研计划项目（GSWSKY-2015-58）；兰州市科技项目（2018-1-109）

Bioinformatic analysis of expression and clinical significance of formyl peptide receptor 1 in gliomas

DONG Qiang¹, XIE Qiqi², HU Jianhong¹, WANG Maolin¹, YUAN Guoqiang³, PAN Yawen^1,3

1. Department of Neurosurgery, Second Hospital of Lanzhou University, Lanzhou 730030;
2. Department of Orthopaedics, Second Hospital of Lanzhou University, Lanzhou 730030;
3. Institute of Neurology, Lanzhou University, Lanzhou 730030, Gansu, China

Received:2018-06-27 Revised:2018-10-14 Online:2018-11-30 Published:2018-11-30

摘要/Abstract

摘要： 目的：分析胶质瘤与正常组织表达差异的基因，筛选和胶质瘤预后相关的关键基因。方法：通过下载GEO（Gene Expression Omnibus）数据库GSE15824原始数据，利用R语言分析正常组织与胶质瘤组织中差异表达的基因，通过生物信息学分析工具（DAVID、String、Cytoscape、oncomine和GEPIA）对差异表达的基因进行生物学功能、蛋白互作网络（PPI）分析及筛选胶质瘤的预后标志物。结果：共筛选出648个差异表达的mRNAs。差异表达mRNAs的生物学功能主要富集于T细胞的激活，调节白细胞的黏附和细胞的迁移。KEGG信号通路分析显示差异基因主要富集于PI3K/Akt，MAPK和钙离子信号通路。FPR1在胶质瘤中明显高表达，其表达水平与胶质瘤的预后呈负相关（Log-rank P < 0.01）。结论：通过基因芯片数据库的分析，FPR1在胶质瘤中高表达，且与患者生存预后相关，为进一步分子机制的研究提供了新思路。

关键词: 胶质瘤, 生物信息学, 基因芯片, 甲酰肽受体1

Abstract: OBJECTIVE: To identify differentially expressed genes in gliomas which were related to their prognosis. METHODS: Raw data of the GEO (Gene Expression Omnibus) database GSE15824 involving normal and glioma tissues were analyzed using R language,and bioinformatics analysis tools (DAVID,String,Cytoscape,oncomine,and GEPIA). Differentially expressed genes were subjected to biological function,protein interaction analysis,and screening for prognostic markers of gliomas. RESULTS: A total of 648 differentially expressed mRNAs, including 471 up-regulated mRNAs and 177 down-regulated mRNAs were screened. Biological functions of these differential genes were mainly enriched in the activation of T cells,and regulation of adhesion of leukocytes and of cell migration. The KEGG signaling pathway was mainly enriched in PI3K/Akt,MAPK,and calcium signaling pathways. Formyl peptide receptor 1 was highly expressed in gliomas,and the expression level was negatively correlated with the prognosis of gliomas (Log-rank P < 0.01). CONCLUSION: Through the analysis of gene expression microarray analysis,FPR1 was highly expressed in gliomas and was related to patients' survival. The observations provide new ideas for further molecular investigations.

Key words: glioma, bioinformatics, gene chip, formyl peptide receptor 1

中图分类号:

R739.41

董强, 解琪琪, 胡建宏, 王茂林, 袁国强, 潘亚文. 基于生物信息学方法分析甲酰肽受体1在胶质瘤中的表达及临床意义[J]. 癌变·畸变·突变, 2018, 30(6): 457-462.

DONG Qiang, XIE Qiqi, HU Jianhong, WANG Maolin, YUAN Guoqiang, PAN Yawen. Bioinformatic analysis of expression and clinical significance of formyl peptide receptor 1 in gliomas[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2018, 30(6): 457-462.

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基于生物信息学方法分析甲酰肽受体1在胶质瘤中的表达及临床意义

Bioinformatic analysis of expression and clinical significance of formyl peptide receptor 1 in gliomas

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