二甲双胍对DEHP和DBP诱导的MCF-7细胞增殖和迁移的抑制作用

doi:10.3969/j.issn.1004-616x.2021.03.007

癌变·畸变·突变 ›› 2021, Vol. 33 ›› Issue (3): 197-202.doi: 10.3969/j.issn.1004-616x.2021.03.007

二甲双胍对DEHP和DBP诱导的MCF-7细胞增殖和迁移的抑制作用

程斌¹, 王新宇², 孙钰¹, 张家泰¹, 董淑英¹

1. 哈尔滨医科大学公共卫生学院环境卫生教研室, 黑龙江哈尔滨 150081;
2. 上海健康医学院, 上海 201318

收稿日期:2020-10-27 修回日期:2021-04-19 出版日期:2021-05-30 发布日期:2021-06-09
通讯作者: 董淑英，E-mail：dong.shuying@163.com E-mail:dong.shuying@163.com
作者简介:程斌，E-mail：chengbin0915@163.com

Inhibition of metformin on phthalate-induced proliferation and migration of MCF-7 breast cancer cells

CHENG Bin¹, WANG Xinyu², SUN Yu¹, ZHANG Jiatai¹, DONG Shuying¹

1. Department of Occupational and Environmental Health Sciences, School of Public Health, Harbin Medical University, Harbin 150081, Heilongjiang;
2. Shanghai University of Medicine & Health Sciences, Shanghai 201318, China

Received:2020-10-27 Revised:2021-04-19 Online:2021-05-30 Published:2021-06-09

摘要/Abstract

摘要： 目的：研究二甲双胍对邻苯二甲酸二（2-乙基己）酯（DEHP）和邻苯二甲酸二正丁酯（DBP）诱导的乳腺癌MCF-7细胞增殖和迁移的抑制作用。方法：设不同浓度的DEHP （20、40、100、200、400 μg/mL）、DBP （0.14、0.28、0.7、1.4、2.8、14、28 μg/L）、二甲双胍（2.5、5、10、20 mg/mL）单独染毒组和溶剂对照组（0.1%二甲基亚砜），并将20 mg/mL二甲双胍分别加入DEHP （100 μg/mL）和DBP （0.7 μg/L）中，采用四甲基噻唑蓝（MTT）实验检测MCF-7细胞的增殖情况，观察细胞形态学变化，用划痕修复实验和Transwell实验检测MCF-7细胞迁移情况。结果：与溶剂对照组相比，DEHP （20~400 μg/mL）、DBP （0.14~28 μg/L）单独染毒组使MCF-7细胞增殖率升高，细胞迁移能力增强，差异均有统计学意义（P < 0.05）；二甲双胍（2.5~20 mg/mL）单独染毒组使MCF-7细胞增殖率明显降低，细胞迁移能力减弱，差异有统计学意义（P < 0.05）。与DEHP和DBP单独染毒组比较，二甲双胍联合DEHP和DBP组，MCF-7细胞的增殖率下降，细胞迁移能力减弱，差异有统计学意义（P < 0.05）。结论：DEHP和DBP可以诱导人乳腺癌MCF-7细胞的增殖和迁移，二甲双胍可以抑制其增殖和迁移作用。

关键词: 邻苯二甲酸酯, 邻苯二甲酸二(2-乙基己)酯, 邻苯二甲酸二正丁酯, 乳腺癌细胞, 二甲双胍

Abstract: OBJECTIVE： This study aims to explore induction of proliferation and migration in MCF- 7 breast cancer cells by phthalates (DEHP，DBP) as well as their inhibition by metformin. METHODS： MCF-7 cells were treated with DEHP (20，40，100，200 and 400 μg/mL)，DBP (0.14，0.28，0.7，1.4，2.8，14，28 μg/L) or metformin (2.5，5，10，20 mg/mL). Untreated cells were used as controls. 20 mg/mL metformin was added to DEHP (100 μg/mL) and DBP (0.7 μg/L) respectively. The effects of DEHP and DBP on induction of proliferation of MCF-7 cells，and inhibitory effects of metformin were detected by MTT assay. Effects of DEHP and DBP on migration of MCF- 7 cells and the inhibitory effects of metformin were detected by the wound healing and Transwell assay. RESULTS： Compared with the control group， MTT and cell migration assays revealed that DEHP (20-400 μg/mL) and DBP (0.14-28 μg/L) treatments significantly increased cell proliferation and migration in MCF-7. On the other hand，metformin (2.5-20 mg/mL) treatment diminished cell proliferation and migration (P < 0.05). Compared with the DEHP and DBP groups， the combined exposures to phthalate (100 μg/mL DEHP， 0.7 μg/L DBP) and metformin (20 mg/mL) diminished cell proliferation and migration of MCF-7 cells. CONCLUSION： The present study demonstrates that metformin inhibited proliferation and migration of MCF-7 breast cancer cells which were induced by DEHP and DBP.

Key words: phthalates, DEHP, DBP, breast cancer cells, metformin

中图分类号:

R737.9

程斌, 王新宇, 孙钰, 张家泰, 董淑英. 二甲双胍对DEHP和DBP诱导的MCF-7细胞增殖和迁移的抑制作用[J]. 癌变·畸变·突变, 2021, 33(3): 197-202.

CHENG Bin, WANG Xinyu, SUN Yu, ZHANG Jiatai, DONG Shuying. Inhibition of metformin on phthalate-induced proliferation and migration of MCF-7 breast cancer cells[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2021, 33(3): 197-202.

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二甲双胍对DEHP和DBP诱导的MCF-7细胞增殖和迁移的抑制作用

Inhibition of metformin on phthalate-induced proliferation and migration of MCF-7 breast cancer cells

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