GMA诱导16HBE恶性转化细胞中LncRNA EMX2OS的表达及意义

doi:10.3969/j.issn.1004-616x.2017.06.004

癌变·畸变·突变 ›› 2017, Vol. 29 ›› Issue (6): 422-426.doi: 10.3969/j.issn.1004-616x.2017.06.004

GMA诱导16HBE恶性转化细胞中LncRNA EMX2OS的表达及意义

王全凯^1,2, 王博深³, 谢广云^1,2, 康同影^1,2, 朱宝立³, 许建宁^1,2

1. 中国疾病预防控制中心职业卫生与中毒控制所, 北京 100050;
2. 中国疾病预防控制中心化学污染与健康安全重点实验室北京 100050;
3. 江苏省疾病预防控制中心, 江苏南京 210009

收稿日期:2017-07-09 修回日期:2017-09-19 出版日期:2017-11-30 发布日期:2017-11-30
通讯作者: 许建宁,E-mail:jnx999@263.net E-mail:jnx999@263.net
作者简介:王全凯,E-mail:kylewang@sina.com;王博深,E-mail:doudouwbs@126.com。
基金资助:
国家自然科学基金资助项目（81673221）

Expression of LncRNA EMX2OS during glycidyl methacrylate-induced malignant transformation of 16HBE cells

WANG Quankai^1,2, WANG Boshen³, XIE Guangyun^1,2, KANG Tongying^1,2, ZHU Baoli³, XU Jianning^1,2

1. National Institute of Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention, Beijing 100050;
2. Key Laboratory of Chemical Safety and Health, Chinese Center for Disease Control and Prevention, Beijing 100050;
3. Jiangsu Center for Disease Control and Prevention, Nanjing 210009, Jiangsu, China

Received:2017-07-09 Revised:2017-09-19 Online:2017-11-30 Published:2017-11-30

摘要/Abstract

摘要： 目的：探讨LncRNA EMX2OS在甲基丙烯酸环氧丙酯（GMA）诱导的16HBE恶性转化细胞中的表达变化及意义。方法：取GMA诱导的第30代16HBE恶性转化细胞及DMSO对照组细胞，采用高通量LncRNA芯片比较两组样本表达谱的差异，并用生物信息学方法筛选出LncRNA EMX2OS及其靶向基因EMX2，采用实时荧光定量PCR（qPCR）分析16HBE恶性转化细胞中LncRNA EMX2OS和EMX2 mRNA的表达水平，并与对照组细胞比较。结果：LncRNA芯片结果显示，与DMSO对照组细胞相比，GMA诱导的16HBE恶性转化细胞中LncRNA EMX2OS上调6.01倍；qPCR结果显示，相对于对照细胞，16HBE恶性转化细胞中LncRNA EMX2OS表达上调3.37倍，EMX2 mRNA上调1.88倍（P < 0.05）。EMX2 mRNA和LncRNA EMX2OS表达的变化趋势一致。结论：GMA可以诱导16HBE细胞LncRNA EMX2OS表达上调，LncRNA EMX2OS可作为GMA诱导的16HBE恶性转化细胞中相关特异分子标志之一。

关键词: 甲基丙烯酸环氧丙酯, 人支气管上皮细胞, LncRNA EMX2OS, EMX2

Abstract: OBJECTIVE:To investigate the expression of long non-coding RNA EMX2OS (LncRNA EMX2OS) during glycidyl methacrylate (GMA)-induced malignant transformation of 16HBE cells. METHODS:Malignantly transformed 16HBE cells (induced by exposure to 8 μg/mL GMA) and solvent control cells (exposed to DMSO) at around the 30th generation were harvested. High throughput LncRNA microarrays were used to detect differences in expression profile of LncRNAs between the two groups of cells. Changes in LncRNAs were screened through strategies such as fold changes and neighboring genes analyses. Real-time fluorescence quantitative PCR (qPCR) and gene chip were applied to measure the expression levels of LncRNA EMX2OS and EMX2,respectively. RESULTS:Based on the result of LncRNA microarrays,expressions of LncRNA EMX2OS was up-regulated by 6.01 fold in the transformed cells campared to the control cells. In addition, qPCR analyses showed that LncRNA EMX2OS increased 3.37 fold and EMX2 increased 1.88 fold change (P < 0.05). The trends of increase in EMX2 and EMX2OS trends were identical. CONCLUSION:GMA induced significant increase in expression of LncRNA EMX2OS of 16HBE cells and the expression can be considered as a specific biomarker which is involved in GMA-induced malignant transformation 16HBE cells.

Key words: glycidyl methacrylate, human bronchial epithelial cells, LncRNA EMX2OS, EMX2

中图分类号:

R730.2

王全凯, 王博深, 谢广云, 康同影, 朱宝立, 许建宁. GMA诱导16HBE恶性转化细胞中LncRNA EMX2OS的表达及意义[J]. 癌变·畸变·突变, 2017, 29(6): 422-426.

WANG Quankai, WANG Boshen, XIE Guangyun, KANG Tongying, ZHU Baoli, XU Jianning. Expression of LncRNA EMX2OS during glycidyl methacrylate-induced malignant transformation of 16HBE cells[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2017, 29(6): 422-426.

参考文献

[1] POLLEUX F. Generation of the cortical area map;emx2 strikes back[J]. Neuron,2004,43(3):295-297.
[2] CECCHI C. Emx2:a gene responsible for cortical development, regionalization and area specification[J]. Gene,2002,291(1/2):1-9.
[3] QIU H,YAN Q,LUO X,et al. EMX2 is downregulated in endometrial cancer and correlated with tumor progression[J]. Int J Gynecol Pathol,2013,32(2):193-198.
[4] LI J,MO M,CHEN Z,et al. Adenoviral delivery of the EMX2 gene suppresses growth in human gastric cancer[J]. PLoS One,2012,7(9):e45970.
[5] YUE D,LI H,CHE J,et al. EMX2 is a predictive marker for adjuvant chemotherapy in lung squamous cell carcinomas[J]. PLoS One,2015,10(7):e132134.
[6] GIROUX L E,HIRATA T,MO M,et al. The homeobox gene EMX2 is a prognostic and predictive marker in malignant pleural mesothelioma[J]. Lung Cancer,2014,85(3):465-471.
[7] NOONAN F C,GOODFELLOW P J,STALOCH L J,et al. Antisense transcripts at the EMX2 locus in human and mouse[J]. Genomics,2003,81(1):58-66.
[8] 阳艳,章汉旺. EMX2、HOXA10与子宫内膜容受性[J]. 生殖与避孕,2006(2):95-98.
[9] TAKAMOCHI K,OH S,MATSUOKA J,et al. Clonality status of multifocal lung adenocarcinomas based on the mutation patterns of EGFR and K-ras[J]. Lung Cancer,2012,75(3):313-320.
[10] 杨敏,许建宁,王全凯,等. 甲基丙烯酸环氧丙酯致人支气管上皮细胞恶性转化研究[J]. 中华预防医学杂志,2009,43(3):187-192.
[11] 董琳,胡洁,王全凯,等. 甲基丙烯酸环氧丙酯致人支气管上皮细胞恶性转化过程中相关基因表达变化的研究[J]. 癌变·畸变·突变,2010,22(4):249-254.
[12] LI K,BLUM Y,VERMA A,et al. A noncoding antisense RNA in tie-1 locus regulates tie-1 function in vivo[J]. Blood,2010,115(1):133-139.
[13] LIU T,HUANG Y,CHEN J,et al. Attenuated ability of BACE1 to cleave the amyloid precursor protein via silencing long noncoding RNA BACE1AS expression[J]. Mol Med Rep,2014,10(3):1275-1281.
[14] FAGHIHI M A,MODARRESI F,KHALIL A M,et al. Expression of a noncoding RNA is elevated in Alzheimer's disease and drives rapid feed-forward regulation of beta-secretase[J]. Nat Med,2008,14(7):723-730.
[15] TRAN V G,COURT F,DUPUTIE A,et al. H19 antisense RNA can up-regulate Igf2 transcription by activation of a novel promoter in mouse myoblasts[J]. PLoS One,2012,7(5):e37923.
[16] SPIGONI G,GEDRESSI C,MALLAMACI A. Regulation of Emx2 expression by antisense transcripts in murine cortico-cerebral precursors[J]. PLoS One,2010,5(1):e8658.
[17] 周吉,沈聪,霍然. Emx2和Emx2OS在小鼠胚胎生殖嵴中的表达[J]. 生殖与避孕,2014(6):437-443.
[18] XU C,YANG M,TIAN J,et al. MALAT-1:a long non-coding RNA and its important 3' end functional motif in colorectal cancer metastasis[J]. Int J Oncol,2011,39(1):169-175.
[19] FRAMPTON A E,CASTELLANO L,COLOMBO T,et al. MicroRNAs cooperatively inhibit a network of tumor suppressor genes to promote pancreatic tumor growth and progression[J]. Gastroenterology,2014,146(1):268-277.
[20] YU T,BACHMAN J,LAI Z C. Evidence for a tumor suppressor role for the large tumor suppressor genes LATS1 and LATS2 in human cancer[J]. Genetics,2013,195(3):1193-1196.
[21] SOH U J,LOW B C. BNIP2 extra long inhibits RhoA and cellular transformation by Lbc RhoGEF via its BCH domain[J]. J Cell Sci,2008,121(Sup 10):1739-1749.
[22] YUE D,LI H,CHE J,et al. EMX2 Is a Predictive marker for adjuvant chemotherapy in lung squamous cell carcinomas[J]. PLoS One,2015,10(7):e132134.

GMA诱导16HBE恶性转化细胞中LncRNA EMX2OS的表达及意义

Expression of LncRNA EMX2OS during glycidyl methacrylate-induced malignant transformation of 16HBE cells

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	李昕苇, 王全凯, 马顺鹏, 王苗, 乌瀚宝栎尔, 顾轶婷, 康同影, 许建宁. 甲基丙烯酸环氧丙酯诱导16HBE细胞恶性转化过程中LncRNA表达特征分析及ceRNA调控网络预测[J]. 癌变·畸变·突变, 2022, 34(1): 1-6.
[2]	王苗, 王全凯, 李昕苇, 马顺鹏, 乌瀚宝栎尔, 许建宁. 甲基丙烯酸环氧丙酯诱导16HBE细胞恶性转化相关m⁶A修饰异常mRNA的分析[J]. 癌变·畸变·突变, 2021, 33(6): 405-409.
[3]	马顺鹏, 王全凯, 王苗, 宋佳阳, 乌瀚宝栎尔, 谢广云, 许建宁. 甲基丙烯酸环氧丙酯诱导16HBE细胞恶性转化过程中细胞凋亡相关lncRNA的筛选及功能研究[J]. 癌变·畸变·突变, 2021, 33(1): 1-5,31.
[4]	蔡颖, 李闰冰, 郑凯, 秦双建, 李柏茹, 张朝晖, 徐新云. PM_2.5染毒HBE细胞前后差异表达的microRNAs及其生物信息学分析[J]. 癌变·畸变·突变, 2020, 32(5): 355-362.
[5]	蔡颖, 郑凯, 秦双建, 李柏茹, 余淑苑, 刘宁, 季佳佳, 张朝晖, 徐新云. 沉默c-fos基因对PM_2.5染毒人支气管上皮细胞癌基因和凋亡相关基因表达的影响[J]. 癌变·畸变·突变, 2020, 32(4): 298-303,308.
[6]	王冰玉, 蔡颖, 郑凯, 谢红卫, 徐新云. PM_2.5对HBE细胞致癌致突变相关基因表达的影响[J]. 癌变·畸变·突变, 2020, 32(1): 33-38,42.
[7]	谢广云, 郭浩然, 王全凯, 马顺鹏, 宋佳阳, 乌瀚宝栎尔, 许建宁. GMA诱导16HBE细胞恶性转化过程中LINC00472的表达及其意义[J]. 癌变·畸变·突变, 2019, 31(6): 449-453.
[8]	王冰玉, 郑凯, 徐新云, 谢红卫, 于军晖, 龙鼎新. PM_2.5对支气管上皮细胞DNA损伤作用研究[J]. 癌变·畸变·突变, 2019, 31(6): 469-473,497.
[9]	王全凯, 谢广云, 马顺鹏, 郭浩然, 乌瀚宝栎尔, 宋佳阳, 许建宁. 甲基丙烯酸环氧丙酯的遗传毒性评价[J]. 癌变·畸变·突变, 2019, 31(6): 479-482.
[10]	刘红梅, 王全凯, 谢广云, 温亚男, 许建宁. LncRNA PCA3在甲基丙烯酸环氧丙酯诱导16HBE恶性转化细胞中的表达及意义[J]. 癌变·畸变·突变, 2016, 28(3): 185-189.
[11]	王全凯, 谢广云, 刘红梅, 许建宁. 甲基丙烯酸环氧丙酯致16HBE细胞恶性转化不同时期METTL9基因甲基化状态分析[J]. 癌变·畸变·突变, 2015, 27(6): 432-436.
[12]	范燕峰, 陈文涛, 王武斌, 杨瑾. 泛素连接酶RING2对苯并[a]芘染毒人支气管上皮细胞周期和P53蛋白表达的影响[J]. 癌变·畸变·突变, 2015, 27(5): 347-352.
[13]	杨瑾, 陈文涛, 范燕峰, 张慧涛. 苯并(a)芘染毒致人支气管上皮细胞的周期改变及BPDE-DNA加合物形成[J]. 癌变·畸变·突变, 2015, 27(2): 86-90.
[14]	陈文涛,范燕峰,张慧涛,杨瑾. 苯并 (a)芘染毒致人支气管上皮细胞和上皮样成纤维细胞周期的变化[J]. 癌变·畸变·突变, 2013, 25(6): 454-456.
[15]	高刚,杨英杰,刘建香,田梅,苏旭. 过表达miR-200家族对α粒子诱发恶性转化人支气管上皮细胞迁移能力的影响[J]. 癌变·畸变·突变, 2013, 25(2): 83-86.