关键词: 子宫肌瘤, 染色体畸变, HMGI-Y基因, RT-PCR

Abstract: BACKGROUND ＆ AIM: To explore the acting mechanism of HMGI-Y gene (high mobility group,a member of HMG family) in uterus myoma etiology through detecting its expression in cytogenetics and molecular genetics with uterus myoma specimen containing the sixth chromosome complex rearrangement. MATERIAL AND METHODS: ①Preparing chromosome specimen of 50 cases uterus myoma and they were detected by cytogenetics analyzing method. They were divided into three experimental groups:the chromosome 6 aberration group, none aberration of the chromosome 6 group, normal chromosome group, calculating the proportion of each group;②mRNA expression of HMGI-Y gene in every group were detected by RT-PCR one step method;3.Protein expression of HMGI-Y gene in every group were detected by immunohistochemistry analysis. RESULTS: ①4 cases in chromosome aberration group, accounting for 8.0 ％(2.0 ％～19.0 ％);8 cases in none aberration group, accounting for 16.0 ％(7.0 ％～29.0 ％);39 cases in normal group,accounting for 76.0 ％.②RT-PCR one step method indicated that mRNA expression of HMGI-Y gene in the sixth chromosome aberration group is higher than that of two other groups markedly; ③immunohistochemistry analysis indicated that protein expression of HMGI-Y gene in the first group is higher than that of the latter groups evidently(P<0.01),there is no difference between them (P>0.05). CONCLUSION: This study illuminated that there was intimate relationship between aberration of the chromosome 6 and uterus myoma, it acted through regulating HMGI-Y gene expression.

Key words: uterus myoma, chromosome aberration, HMGI-Y gene, RT-PCR