癌变·畸变·突变 ›› 2004, Vol. 16 ›› Issue (1): 24-26.doi: 10.3969/j.issn.1004-616x.2004.01.007

• 论著 • 上一篇    下一篇

低剂量甲氨蝶呤对DNA的损伤及甲酰四氢叶酸对其保护作用

杨建一;李　莉;彭　芸;杜圣家   

  1. 山西医科大学基础医学院，山西　太原　 030001
  • 收稿日期:2003-08-20 修回日期:2003-09-25 出版日期:2004-01-30 发布日期:2004-01-30
  • 通讯作者: 杨建一

DNA Damage Caused by low Dose Methotrexate and Protective Effect of Folinic acid

YANG Jian-yi;LI Li;PENG Yun;et al   

  1. Shanxi Medical University, Taiyuan 030001，China
  • Received:2003-08-20 Revised:2003-09-25 Online:2004-01-30 Published:2004-01-30
  • Contact: YANG Jian-yi

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摘要： 背景与目的：研究多次低剂量应用甲氨蝶呤所致BALB/c小鼠淋巴细胞及骨髓细胞DNA的损伤，并探讨甲酰四氢叶酸对其损伤的保护作用。材料与方法：应用甲氨蝶呤0.5 mg/kg腹腔注射，每周1次，共8周；用单细胞凝胶电泳方法检测其外周血淋巴细胞及骨髓细胞DNA的损伤情况。结果：单用甲氨蝶呤组细胞尾长及拖尾频率与对照组相比均有显著差异，而同时应用等量的甲酰四氢叶酸，其细胞尾长及拖尾频率与对照组相比无显著差异。结论：SCGE技术可敏感地检测多次应用低剂量甲氨蝶呤造成的DNA损伤，并能反映甲酰四氢叶酸对甲氨蝶呤所致的DNA损伤的保护作用。

关键词: 甲氨蝶呤, 甲酰四氢叶酸, 单细胞凝胶电泳, DNA损伤

Abstract: BACKGROUND ＆ AIM: To study the DNA damage of BALB/c mice caused by multiple treatment with low－dose methotrexate (MTX)and the protective effect of folinic acid(FA). MATERIAL AND METHODS:After ip MTX at dose of 0.5 mg/kg once a week for 8 weeks, DNA damage of peripheral lymphocytes and bone marrow cells were examined by single cell gel electrophoresis(SCGE). RESULTS: There were signifi cant differences of the tail length and the frequency of tailing cells between MTX and the control. However, there were no signifi cant differences between MTX＋FA and the control. CONCLUSION: SCGE is a rapid, simple and sensitive technique for detecting DNA damage caused by low dose methotrexate and folinic acid could protect peripheral lymphocytes and bone marrow cells DNA from damage induced by MTX.

Key words: methotrexate, folinic acid, single cell gel electrophoresis(SCGE), DNA damage

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