Abstract: Purpose and Methods : Using immunohisthocemical assay , the expression of cyclin D1 protein of lithium carbonate on the development of rat hepatic carcinoma induced by aflatoxin B1 and it s significance were studied. Results : The expression of cyclin D1 occurred in the early stage (the 6th week) and significantly increased in the 10th week. The positive rate of positive cont rol group (B) was the highest and that of group t reated with Li2CO3 simultaneously (C) and group pre2t reated with Li2CO3 . (D) significantly decreased , but group C > group D. Conclusion : It suggested that Li2CO3 has the effect on anti2expression of cyclin D1 in aflatoxin B1 induced rat hepatic carcinoma , and can rest rain cell continuous roliferation , so it may be anticarcinogenic ; and that the immunohistochemical assay of cyclin D1 may help early diagnosis and monitoring.

Key words: cyclin D1, Li2CO3, AFB1, rat hepatic carcinoma