Carcinogenesis, Teratogenesis & Mutagenesis ›› 2007, Vol. 19 ›› Issue (4): 331-334.doi: 10.3969/j.issn.1004-616x.2007.04.019

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Effects of Experiment Error Factors on Micronucleus Frequency Scoring in Cytokinesis_Blocked Lymphocyte

SUN Hua_ming, CHEN Guo_hua, ZHOU Li_jun, ZHOU Zi_yuan, CAO Jia   

  1. Department of Hygiene and Toxicology, College of Preventive Medicine, Third Military Medical University,Chongqing
  • Received:2006-07-13 Revised:2007-05-09 Online:2007-07-30 Published:2007-07-30

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Abstract: BACKGROUND ＆ AIM：To study the effect on MN frequency identification caused by different skill level, staining time and scoring time. MATERIALS AND METHODS: A blood sample was collected from a healthy 30_year_old male. Lymphocytes was isolated and then cultured in RPMI medium in triplicates. Two of the 3 cultured groups were then exposed to 1Gy or 2 Gy gamma_rays and the other one was control. Parallel scoring spots (a and b) in same slide was made according to CBMN assay method, 2 simultaneously parepared slides of each group was stained by Giemsa at 2 different times，and then 6 scorers with different skill levels scored the MN frequency. RESULTS: Variation of MN frequency scored by scorers with different skill levels was not significant.The MN frequency scoring for spot a and b at different times showed no significant difference among different scorers. The observed endpoints, MNed、MNi、NPB and NDI, stained at different times, were not different significantly either. CONCLUSION: Skill level, staining time or scoring time did not show significant interference on estimation of the dose_effect relationship between MN frequency and radiation dose.

Key words: micronucleus test, skill level, staining time, scoring time