Abstract: OBJECTIVE: To determine the association of hCHK2 rs2278022, rs2602431, and rs2970077 polymorphism and haplotypes with the susceptibility of esophageal cancer in Kazakh and Han nationality in Xinjiang Uygur Autonomous Region. METHODS: A molecular epidemiology method was carried out of 239 cases of esophageal cancer(132 Kazakh nationality, 107 Han nationality)and 513 controls(309 Kazakh nationality, 204 Han nationality)in Xinjiang area. The polymorphism of hCHK2 gene at rs2278022, rs2602431 and rs2970077 position was analyzed by polymerase chain reaction-ligase detection reaction (PCR-LDR) methods. Haplotypes were estimated by the SHEsis software. The haplotype distribution statistical differences of the genotype and haplotype frequencies between the case group and the control group were estimated. RESULTS: No significant difference was observed in the frequency of hCHK2 gene at rs2278022, rs2602431 and rs2970077 position genotypes and allele genes between the cases and controls in Kazakh and Han nationality(P>0.05). In Kazakh and Han nationality, the distribution of haplotypes was not significantly different between esophageal cancer cases and controls(P>0.05); CONCLUSION: The polymorphism of hCHK2 gene at rs2278022, rs2602431 and rs2970077 position and haplotypes are unlikely to be associated with the susceptibility to esophageal cancer in Kazakh and Han nationality.

Key words: hCHK2 gene, single nucleotide polymorphism, haplotype, esophageal cancer, Kazakh nationality