B7-H1基因rs4143815位点多态性与结直肠癌发病风险的关联研究

doi:10.3969/j.issn.1004-616x.2019.06.004

Abstract

Abstract: OBJECTIVE: B7-H1 is a target of tumor immunotherapy. A polymorphism rs4143815C/G in the 3'-untranslated region of B7-H1 can affect its binding to miR-570,which in turn influences the expression of B7-H1. The aim of this study was to investigate the association between the rs4143815 polymorphism and risk of colorectal cancer (CRC). METHODS: Peripheral venous blood was collected from 215 patients with CRC and 236 age- and gender-matched healthy controls. Tumor tissues and adjacent normal tissues were collected from 65 patients with CRC. The rs4143815 polymorphism was genotyped by direct sequencing and B7-H1 mRNA levels were examined by quantitative PCR. The association between the rs4143815 polymorphism in the B7-H1 gene and the risk of CRC was analyzed using chi-square test. RESULTS: Three genotypes of the rs4143815 polymorphism in the B7-H1 gene were detected in both cases and controls,that is CC,CG and GG genotype. The B7-H1 mRNA expression was observed in CRC tissues and adjacent normal tissues. The rs4143815 GG and CG/GG genotypes were associated with a significantly increased risk of CRC compared with the CC genotype[GG vs. CC:adjusted OR=1.99,95%CI (1.19,3.34),P=0.008;CG/GG vs. CC:adjust OR=1.58,95%CI (1.06,2.36),P=0.02]. Similarly,the G allele was associated with a significantly increased risk of CRC compared with the C allele[adjusted OR=1.48,95%CI(1.14,1.93),P=0.004]. The expression of B7-H1 mRNA in CRC tissues was significantly higher than that in adjacent tissues (P=0.02). Moreover,the B7-H1 mRNA level in the rs4143815 GG genotype carriers was significantly higher than that in the rs4143815 CC genotype carriers (P=0.03). CONCLUSION: The rs4143815 GG genotype in the B7-H1 gene may increase the expression of B7-H1 mRNA and result in an increased risk of CRC.

Key words: B7-H1, miR-570, single nucleotide polymorphism, colorectal cancer

CLC Number:

R730.2

MA Xiaobiao, ZHANG Qi, PAN Dingguo. Association between rs4143815 polymorphism in the B7-H1 gene and risk of colorectal cancer[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2019, 31(6): 440-443,448.

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Association between rs4143815 polymorphism in the B7-H1 gene and risk of colorectal cancer

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