外周血微核试验检测可降解生物材料补片的遗传毒性

doi:10.3969/j.issn.1004-616x.2023.05.010

Abstract

Abstract: OBJECTIVE:Based on flow cytometry,genotoxicity of biodegradable patch was investigated by studying the induction of micronuclei in peripheral reticulocytes (MN-RET) and mature erythrocytes (MN-NCE) in mice under different administration conditions. METHODS: For the short-term administration condition, mice were intraperitoneally injected with vehicle control,test solution,and cyclophosphamide solution (25,50 mg/kg) once a day for 3 days. Then,peripheral blood samples were collected,and the micronucleus were detected by flow cytometry. For the long-term administration condition, mice were continuous administrated for 14 days. Then,peripheral blood samples were collected,and spleens were enucleated. Micronucleus frequencies as well as splenic cell apoptosis were detected by flow cytometry. In addition, spleens were histopathologically examined after hematoxylin-eosin staining (HE). RESULTS: There was no significant differences in the percentage of MN-RET and MN-NCE in both treatment and control groups under the two different administration conditions (P> 0.05). In addition, there was no significant difference in the apoptosis rate of spleen cells between the two groups (P>0.05),and there were no significant pathological changes in the spleen tissue. On the other hand, significant and positive results were observed with the cyclophosphamide groups. CONCLUSION: The micronucleus test results show that the biodegradable patches had no potential genotoxic risk. In addition,the selection and analysis of MN-RET and MN-NCE in mouse peripheral blood under different dosing cycle conditions was useful in detecting genotoxic risk of chemicals.

Key words: flow cytometry, micronucleus test, genotoxicity, biodegradable patches

CLC Number:

R994.6

WANG Guowei, SUN Xiaoxia, CHE Guoxi, GAI Xiaoxiao, QU Qiujin, WANG Yan, WANG Xiaofei, DU Xiaodan, LIU Chenghu. Genotoxicity evaluation of biodegradable patches using the micronucleus test in peripheral blood of mice[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2023, 35(5): 387-391.

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Genotoxicity evaluation of biodegradable patches using the micronucleus test in peripheral blood of mice

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