The f requency of 　Human sperm chromosome aneuploidy was assessed from 10 normal and health adult s by using the method of FISH. Sperm heads were decondensed with dithilthreitol (DTT) and lithium diiodosalicylate (LIS) , then the sperm nuclei were hybridized with biotin labeled alpha satellite X chromosome specific DNA probe (DXZ1) in 37 ℃ overnight . The hybridization signals were detected with Cy32St reptavidin. The red hybridization signals in X sperms were clearly visible under the Nikon fluorescence microscope. The hybridization efficiency was 48. 62 % ( rance 47. 54 % to 49. 36 %) . A total 55453 X sperm nuclei were observed. The frequency of disomic sperms was 0. 06 %. There was no significant difference observed in disomic frequency of X sperms between ours and that reported by former authors. The information provided by this paper is valuable for further studies on men exposed occupationally or environmentally to potential aneuploidogenic agents.

In order to study the expression of bcl - 2 , p53 protein in laryngeal squmaous cell carcinoma (LSCC) , the prognostic significance , their expression in 53 cases with laryngeal squmaous cell carcinoma were determinded by immunohistochemical LSAB method. The result s showed that : (1) In 47. 17 %LSCC , the expression of mutant p53 protein were positive. Meanwhile , mutant p53 protein was also expressed in some dysplastic epitheliumes near the cancer tissues. These epithelial cells near the tumor in which p53 gene has been altered , probably are the source of tumor recurrence , the expression of mutant p53 protein may be the early event s in the development of LSCC. The p53 protein expression was associated with low histological grade of differentiation , high mitotic count ( ( P < 0. 05 , P < 0. 01) . The necrosis in tumors with the p53 positive was higer than those with the P53 negative LSCC( P < 0. 01) ,patient s with p53 - positive LSCC had shorter survival times than patient s with p53 - negative LSCC. p53 protein expression was not associated with node status and age , is valuable in determinding the degree of malignancy , predicting tumor differentiation (2) In LSCCs , bcl - 2 expression was consistently low and related with low mitotic count and younge age ( P < 0. 05 , P < 0. 05) ,and bcl - 2 expression was not related with histological grade of differentiation , necrosis , node status and survival time.

To make certaion stable experimental parameters about FADU in detection of CHL DNA breaks , temperature of DNA unwinding and concent ration of unwinding alkaline solution NaOH were modified through FADU on CHL cells. According to Birnboim’s criterion , unwinding temperature first 0 ℃/ 30 min →then 5 ℃/ 60 min and NaOH 0. 4N were selected as better conditions. The reliability of these experimental parameters was established by detection of peroxidative adaptive response and antiperoxidation on CHL DNA breaks induced by H2O2 with the improved FADU.

Two aneugens : vinblastine (Vin) and colchicine (Col) ,and two clastogens : mitomycin C (MMC) and cyclophosphamide (CP) , were applied to Chinese hamster lung (V79) cells respectively in vit ro for micronucleus test . The result s showed that the increases of incidence of multinucleated cells ( Imu) and that of micronucleated cells ( Imi ) in response to aneugens and clastogens were in different sensitivities. At relatively low concent rations , Vin and Col induced only Imu (but not Imi) , while MMC induced only Imi ; At higher concent rations , both Imu and Imi increased in a concent ration dependent manner , however , Vin and Col induced multinuclei predominantly with the ratios of Imu to Imi 2. 0 - 3. 5 ,while MMC and CP induced micronuclei mainly , with Imu/ Imi of 0. 08 - 0. 28. This intensity of difference in Imu/ Imi was much greater than that in areas of micronuclei between aneugens and clastogens. The result s indicate that in micronucleus test in vit ro , observation of both Imu and Imi might be valuable for distinguishing aneugens f rom clastogens.

Epristeride is an uncompetitive 5α2reductase inhibitor , using for t reatment of benign prostatic hyerplasia. Fertility study on epristeride was carried out in SD rat s. Epristeride was given to male rat s for 9 weeks before mating performance at dose level of 3 ,15 ,75 mg/ kg. Body weight gain and food intake were similar to cont rol. No influence of epristeride in mating performance was observed , but showing slight less of pregnancy rate. No external , visceral and skeletal anomalies were found in fetuses. A little bit of increase in abnormal sperm morphology was observed f rom epristeride2t reated mice at high dose , but there is no statistical significant . Epristeride did not inhibit the elect rically evoked cont ractions of mouse vas deferens. In conclusion ,there is a wide range of safety in reproduction for Epristeride.

The influence of a long2term exposure to 70 gasoline on chromosomes and micronuclei in bone marrow cells of mice was investigated. The animals were exposed to 70 gasoline at low (250mg/ m3) , medial (500mg/ m3) and high (1000mg/ m3) doses for 30 、60 、90d. After exposure for 90d (at high dose/ 1000mg/ m3) , the f requency of CA was significantly higher than that in the negative cont rol group. However , after exposure of r 60d (at high dose) and 90d (at mid/ high doses) , the f requency of micronuclei in polychromatic erythrocytes was significantly higher than that in the negative cont rol group. The data suggested that chronic exposure to gasoline may be hazardous to chromosome in bone marrow cells of mice.

Through observations on the micronucleus formation in human lymphocytes in vitro and bone marrow of mice , the induced mutagenesis effect s of low dose gamma radiation combining cigarette smoking were studied. Cigarette smoking (0. 81cigarette/ ml) and low dose gamma radiation (0. 251. 0Gy) induced signficantly micronulei formation in human lymphocytes ( P < 0. 01) respectively and the significant dose2response was found. The significant higher rate of micronuleus than that of cont rol group was observed when low dose gamma radiation combining cigarette smoking was used to t reat the human lymphocytes. When low dose gamma radiation combining high concent ration cigarette smokeing (1. 5cigarette/ ml) was used to t read the mice , the significant effect s of induction and interaction on the micronucleus formation of mice bone marrow were also observed. The result sindicated that hereditary substance aberrations would be enhanced when low dose gamma radiation combined cigarette smoking.

The purpose of this study was to investigate the inhibition effect of DNA synthesis and the AFP secretion on BEL - 7402 human hepatocarcinoma cells in the presence of linolenic acid ,linoleic acid and oleic acid which having the same carbon chains. The AFP concent ration was determinated with radioimmunoassay ,while the DNA synthesis was studied by the 3 H - TdR incorporation (CPM) . The result sindicated that three f ree fatty acids could inhibite the DNA synthesis of human hepatocarcinoma cells. The effect s of linolenic acid and linoleic acid( P < 0. 01) were significant ,which were st ronger than that of oleic acid ( P > 0. 05) . 40 - 50μg/ ml concent ration of linolenic acid could inhibite the AFP secretion of hepatocarcinoma cells obviously compare with the blank ( P < 0. 05) . The same concent ration of linoleic acid and oleic acid had no difference compare with the blank ( P > 0. 05) . The conclusion showed that there was a relationship between the molecular st ructure and the inhibition effect s of three fatty acids containing different numbers of double - bond.

The purpose of this paper is to study the effect of VitB6 on f requency of sister chromatid exchange (SCE) in cells f rom human and animals by using in Vitro and in Vivo assay. In vit ro assay , the frequency of SCE was markedly increased in both cells f rom human lymphocytes and the Chinese hamster lung cell line (CHL) , which were exposed to the VitB6 in various doses.A dose response was presented. But in Vivo assay , the statistic difference between the SCE rates of mouse bone marrow cells in test group and in that of cont rol group was not observed. In Vivo assay , it seems that the VitB6 may be not a muatgen.

This paper reported the research results of mutagenesis of oral corn pollen haw liquor (OCPHL) assayed with Ames test , bone marrow micronucleus test in mouse , lymphocyte chromosomal aberration test in human beings. and aberration of sperm test in mouse. The results showed that the OCPHL with 3 doses were not more than the negative controls reversion frequencies in strain TA97 , TA98 , TA100 with or without S9 in the Ames test , It did not raise the frequency of micronucleus , chromosomal aberration and sperm malformation. The results showed that the OCPHL induced no mutagenicity.

In this experiment , green tea were used as detected material ,alum as the mutagen ,the f requency of micronucleus as the index of DNA damage. The result was that feed containing 10 % green tea decrease the f requency of micronucleus f rom 7167 ±1197 ‰to 3150 ±1105 ‰( P <0101) . The result showed that the green tea could inhibit DNA damage induced by alum.