关键词: 雷公藤多甙, 骨髓微核, 精子畸形, 染色体畸变, 联会复合体

Abstract: BACKGROUND AND AIM: In order to detect glycosides of tripterygium wilfordii Hook. f(GTW)male mice genital system toxicity,which can provid rationale and evidence to use safely during the period of clinical treatment. MATERIALS AND METHODS:30 Kunming male mice were randomly divided into five groups with 6 mice in each,three dosage groups (antifertility_dosage groups [10 mg/(kg·d)], medium_dosage groups[20 mg/(kg·d)] and remedy_dosage groups[30 mg/(kg·d)], one negative control and one positive control group.This essay introducts micronucleus of bone marrow,sperm deformity,chromosome aberration,and analyze of synaptonemal complex, approaching effect of GTW to male mice in genesis toxicity. RESULTS: Micronuclei of bone marrow cell indicates 20 mg/(kg·d) group and 30 mg/(kg·d) group of GTW generate somatocyte inherent toxicity, were significantly higher than that of negative control. Every_dosage group of GTW cause sperm deformity, do harm to primary spermatocyte chromosome aberration and synaptonemal complex, were significantly higher than that of negative control. CONCLUSION: GTW had somatocyte inherent and reproductive cell toxicity, reproductive cell was higher sensitivity than somatocyte ,which cann′t apply under large_dosage for a long time as the antifertility or the therapy drug.

Key words: glycosides of tripterygium wilfordii hook.f, micronuclei of bone marrow cell, sperm deformity, chromosome aberration, synaptonemal complex