维吾尔族霍奇金淋巴瘤与HLA-DRB1、DPA1基因多态性的相关性研究

doi:10.3969/j.issn.1004-616x.2017.02.008

癌变·畸变·突变 ›› 2017, Vol. 29 ›› Issue (2): 115-120.doi: 10.3969/j.issn.1004-616x.2017.02.008

维吾尔族霍奇金淋巴瘤与HLA-DRB1、DPA1基因多态性的相关性研究

张海燕, 李迅

新疆医科大学附属肿瘤医院乳腺内科, 新疆乌鲁木齐 830011

收稿日期:2016-11-23 修回日期:2017-02-22 出版日期:2017-03-31 发布日期:2017-03-31
通讯作者: 李迅,E-mail:smxlixun@sina.com E-mail:smxlixun@sina.com
作者简介:张海燕,E-mail:764584078@qq.cm。
基金资助:
新疆自治区优秀青年科技创新人才培养项目（2014721044）

HLA-DRB1 and DPA1 gene polymorphisms in Hodgkin's lymphoma of Uighurs

ZHANG Haiyan, LI Xun

Breast Medicine, Affiliated Cancer Hospital of Xinjiang Medical University, Urumqi 830011, Xinjiang, China

Received:2016-11-23 Revised:2017-02-22 Online:2017-03-31 Published:2017-03-31

摘要/Abstract

摘要：

目的：探索人类白细胞抗原（HLA）DRB1和DPA1低分辨等位基因型与维吾尔族霍奇金淋巴瘤（HL）易感性的关系。方法：采用病例-对照研究（1∶2）和DNA直接测序分型（SBT）法，对40例维吾尔族HL患者和80名健康体检者进行HLA-DRB1及DPA1基因分型，分析其与HL发病的相关性。结果：对于HLA-DRB1和DPA1基因，病例组分别检出12和3个低分辨等位基因，对照组中分别检出13和4个低分辨等位基因；HLA-DRB1、DPA1基因座位上等位基因频率分布均满足Hardy-Weninberg遗传平衡检验（P > 0.05）。维吾尔族HL中HLA-DRB1*15、DPA1*03、DPA1*02-DRB1*13基因表达均高于维吾尔族健康对照组（P均<0.05）；而HLA-DRB1*07基因频率低于维吾尔族健康对照组（P < 0.05）。结论：HLA-DRB1、DPA1基因多态性可能与维吾尔族HL的发病存在关联，在揭示HL的发病机制方面有研究价值。

关键词: 霍奇金淋巴瘤, 维吾尔族, 人类白细胞抗原, 基因频率

Abstract:

OBJECTIVE: To explore the relationship between the human leukocyte antigen (HLA) DRB1,DPA1 low resolution type alleles and susceptibility of Uighurs to develop Hodgkin's lymphomas (HL). METHODS: Using a case-control study design,DNA direct sequencing classification method (SBT) was used to detect alleles in HLA-DRB1 and expression of HLA-DPA1 gene among 40 cases of Uighur patients and 80 healthy controls. The HLA-DRB1 and DPA1 allele frequencies between the two groups were compared. The correlation between them with the pathogenesis of HL was analyzed. RESULTS: HLA-DRB1 and DPA1 low resolution alleles were detected 12,3 types in the case group and 13,4 types in the control group,the frequency distribution of which met the Hardy-Weninberg genetic balance test (P > 0.05). The results show that HLA-DRB1*15,DPA1*03 and DPA1*02-DRB1*13 gene frequencies were significantly more frequent in the Uighur patients than the controls (P=0.016,OR=3.476;P=0.002,OR=4.248;P=0.037,OR=3.028,respectively);HLA-DRB1*07 gene frequencies between the two groups were statistically significant (P=0.025,OR=0.314). CONCLUSION: HLA-DRB1,DPA1 gene polymorphism may be closely associated with the onset of HL among Uighurs and with pathogenesis of HL.

Key words: Hodgkin's lymphoma, Uygur, human leukocyte antigen, gene frequency

中图分类号:

R73-34

张海燕, 李迅. 维吾尔族霍奇金淋巴瘤与HLA-DRB1、DPA1基因多态性的相关性研究[J]. 癌变·畸变·突变, 2017, 29(2): 115-120.

ZHANG Haiyan, LI Xun. HLA-DRB1 and DPA1 gene polymorphisms in Hodgkin's lymphoma of Uighurs[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2017, 29(2): 115-120.

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维吾尔族霍奇金淋巴瘤与HLA-DRB1、DPA1基因多态性的相关性研究

HLA-DRB1 and DPA1 gene polymorphisms in Hodgkin's lymphoma of Uighurs

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