Carcinogenesis, Teratogenesis & Mutagenesis ›› 2005, Vol. 17 ›› Issue (1): 27-29.doi: 10.3969/j.issn.1004-616x.2005.01.009

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Test of Combined-toxicity of Halids to Dunaliella Salina

HUANG Xiao-juan;SHEN Luo-fu;JIANG Jian-guo;et al   

  1. College of Food and Biological Engineering，South China University of Technology，Guangzhou 510640, China
  • Received:2004-05-17 Revised:2004-08-10 Online:2005-01-30 Published:2005-01-30
  • Contact: HUANG Xiao-juan

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Abstract: BACKGROUND ＆ AIM: According to the standard methods of the Organization for Economic Co-operation and Development (OECD) for algal growth inhibition test, the combined-toxicitiy test of three kinds of halid-mixtures to Dunaliella salina was conducted. MATERIAL AND METHODS: Four groups of the tests were set by combining any two of CHCl3, CCl4, CHBr3 and all the three compounds, another group was a control. Concentrations were set according to the National drinking water health standard (GB5749-1985) and D. salina was cultivated in these groups. Cell counting was conducted after 48 h. RESULTS: The EC50 of each group was obtained, and the combined-toxicity's addition-indexes (AI) of the groups were all among －0.1～0.1, which indicated that the toxicity of these compounds to D.salina was additive effect. CONCLUSION: As a tested organism, D. salina has a wide applied potential in the area of toxicology.

Key words: Dunaliella salina, combined toxicity, halids