癌变·畸变·突变 ›› 1998, Vol. 10 ›› Issue (3): 160-163.doi: 10.3969/j.issn.1004-616x.1998.03.009

• 论著 • 上一篇    下一篇

喉癌与蛋白激酶C 及其抑制物的关系

任　重　马　明　宗志宏   

  1. 中国医科大学附属第一临床学院耳鼻咽喉科　沈阳　110001
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:1998-05-30 发布日期:1998-05-30

THE RESEARCH OF THE RELATION BETWEEN LARYNX CANCER AND PROTEIN KINASE C ( PKC) AS WELL AS ITS INHIBITOR( PKCI)

Ren Zhong , Ma Ming , Zong Zhinhong   

  1. Dept of Otolaryngology The First Affiliated , China Medical University S henyang 110001
  • Received:1900-01-01 Revised:1900-01-01 Online:1998-05-30 Published:1998-05-30

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摘要： 为了探讨喉癌与蛋白激酶C(PKC) 及其抑制物( PKCI) 的关系。应用Takai 蛋白浓度梯度,通过同位素放射性活性测定PKC 活性及测定PKCI 对PKC 活性抑制的百分数。结果为癌组织胞浆中PKC 比活性(6163 ±0164pmol·mg - 1·min - 1) 显著高于正常组织胞浆中PKC 比活性(4. 73 ±0. 54pmol·mg - 1·min - 1 ) ,癌组织胞浆中PKC 抑制活性(33. 6 %±7. 64 %) 显著低于正常组织胞浆中PKCI 抑制活性(61. 3 % ±7. 91 %) 。结果提示癌组织中PKC 活性升高很可能是被癌基因激活,而PKCI 活性降低导致对PKC 调控能力下降。

关键词: 喉肿瘤, 蛋白激酶C, 蛋白激酶C 抑制物

Abstract: purpose to explore the relation between larynx cancer and protein kinase C(PKC) as well as it s inhibitor (PKCI) . Method :using Takai protein concent ration gradient ,We measured the activity of PKC and the inhibitory percent of PKCI to PKC activity through measuring the activity of radioistope. result :the PKC activity in cellular plasma of canser tissue (6. 63 ±0. 64pmol·mg - 1·in - 1) was apparently higher than that in cellular plasma of normal tissue (4. 73 ±0. 54pmol·mg - 1·min - 1) . The PKCI inhibitory activity in cellular plasma of cancer tissue (3316 % ±7. 64 %) was apparently lower than that in cellular plasma of normal tissue (61. 3 ±7. 91 %) . conclusion :this result indicated that the rising of PKC activity of cancer tissue might be provoked by oncogene ,and the lowering of PKCI activity was due to decreased regulative ability to PKC.

Key words: Larynx cancer, Protein Kinase C(PKC), Protein Kinase C’s inhibitor (PKCI)

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