关键词: 肿瘤细胞, 着丝粒点, 非整倍性畸变

Abstract: BACKGROUND AND AIM: To study the relation between the centromeric dots(Cd)variation in JEG_3 cells and PA_1 cells and the chromosome aneuploidy aberration。 MATERIALS AND METHODS: Chromosome of tumor cells were prepared with conventional method，chromosome of normal embryonic villi were treated directly. A Cd_NOR in_phase silver dye analysis technique modified by this laboratory was used to study centromeric dots(Cd) variation of JEG_3 cells and PA_1 cells. RESULTS: Frequency of Cd loss in JEG_3 cells was 1.43％, frequency of Cd duplication delay was 0.21％, frequency of small Cd was 1.52％ and that of Cd_NOR fusion was 1.16％。 Frequencies of Cd loss and Cd_NOR fusion in JEG_3 cells were significantly higher than those of normal embryonic villi(P<0.0125). Frequency of Cd duplication delay and small Cd showed no difference between JEG_3 cells and normal embryonic villi cells. Frequency of Cd loss in PA_1 cells was 1.22％, of Cd duplication delay was 0.95％, of small Cd was 1.81％,and that of Cd_NOR fusion was 1.03％。 Frequencies of Cd loss, Cd duplication delay and Cd_NOR fusion in PA_1 cells were significantly higher than those of normal embryonic villi cells(P<0.0125). Frequency of small Cd showed no difference between PA_1 cells and normal embryonic villi. CONCLUSION: Cd loss and Cd_NOR fusion might be related with aneuploidy aberration of JEG_3 cells.Cd loss,Cd duplication delay and Cd_NOR fusion might be related with aneuploidy aberration of PA_1 cells.

Key words: tumor cells, centromeric dots, aneuploidy aberration